| 质粒名称: | pCAX APP Swe/Ind |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 30145 |
| 存储实验室: | Dennis Selkoe, Tracy Young-Pearse |
|
载体骨架基本信息 |
|
| 载体名称: | pCAX |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 4731 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | human APP 695 Swedish/Indiana mutation,amyloid precursor protein |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 2000 bp |
| 基因突变: | 瑞典和印第安纳；K595N、M596L和V642F |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | EcoRV（克隆期间销毁） |
| 3' 克隆位点: | EcoRV(未破坏) |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pCAX APP Swe/Ind was a gift from Dennis Selkoe & Tracy Young-Pearse (Addgene plasmid # 30145 ; http://n2t.net/addgene:30145 ; RRID:Addgene_30145) |
| 参考文献部分: | A critical function for beta-amyloid precursor protein in neuronal migration revealed by in utero RNA interference. Young-Pearse TL, Bai J, Chang R, Zheng JB, LoTurco JJ, Selkoe DJ. J Neurosci. 2007 Dec 26. 27(52):14459-69. 10.1523/JNEUROSCI.4701-07.2007 PubMed 18160654 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCAX APP Swe/Ind 6816 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pCAX APP Swe/Ind
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6816)
AUTHORS Young-Pearse TL, Bai J, Chang R, Zheng JB, LoTurco JJ, Selkoe DJ
TITLE A critical function for beta-amyloid precursor protein in neuronal
migration revealed by in utero RNA interference.
JOURNAL J Neurosci. 2007 Dec 26. 27(52):14459-69.
PUBMED 18160654
REFERENCE 2 (bases 1 to 6816)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6816
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 4..383
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
intron 637..1653
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
primer_bind 1577..1599
/label=pCAGGS-5
/note="Chimeric intron in CAG promoter, forward primer"
primer_bind 1661..1680
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
CDS complement(2053..2064)
/codon_start=1
/gene="cspA"
/product="translation enhancing element for E. coli (Qing
et al., 2004)"
/label=TEE
/translation="NHKV"
CDS 2544..2564
/codon_start=1
/product="6xHis affinity tag"
/label=7xHis
/translation="HHHHHHH"
primer_bind complement(3837..3856)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_site 3902..3957
/label=-beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal"
primer_bind complement(3956..3975)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
primer_bind complement(4318..4334)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4318..4334)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4331..4353)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4342..4358
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4366..4396)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4411..4432
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 4491..4686
/label=SV40 promoter
/note="SV40 early promoter"
rep_origin 4537..4672
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 4599..4618
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
polyA_signal 4692..4826
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4729..4748)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 4783..4802
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(4894..4911)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5065..5653)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5145..5164)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5824..6684)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6447..6466
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6685..6789)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
481 cgatgggggc gggggggggg ggggggcgcg gggcggggcg gggcggggcg grgrggggcg
541 gcggcrgcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
601 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc
661 cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta
721 ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt
781 taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc
841 cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc
901 gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt
961 gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg
1021 ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg kgggggggtg agcagggggt
1081 gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc ctccccgagt tgctgagcac
1141 ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc
1201 ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc cggggagggc
1261 tcgggggagg ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc ggcgagccgc
1321 agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt gtcccaaatc
1381 tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag
1441 cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc
1501 cgtccccttc tccctctcca gcctcggggc tgtccgcggg gggacggctg ccttcggggg
1561 ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag agcctctgct
1621 aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttattgtg
1681 ctgtctcatc attttggcaa agaattcagg taccgatagc ttgccatgct gcccggtttg
1741 gcactgctcc tgctggccgc ctggacggct cgggcgctgg aggtacccac tgatggtaat
1801 gctggcctgc tggctgaacc ccagattgcc atgttctgtg gcagactgaa catgcacatg
1861 aatgtccaga atgggaagtg ggattcagat ccatcaggga ccaaaacctg cattgatacc
1921 aaggaaggca tcctgcagta ttgccaagaa gtctaccctg aactgcagat caccaatgtg
1981 gtagaagcca accaaccagt gaccatccag aactggtgca agcggggccg caagcagtgc
2041 aagacccatc cccactttgt gattccctac cgctgcttag ttggtgagtt tgtaagtgat
2101 gcccttctcg ttcctgacaa gtgcaaattc ttacaccagg agaggatgga tgtttgcgaa
2161 actcatcttc actggcacac cgtcgccaaa gagacatgca gtgagaagag taccaacttg
2221 catgactacg gcatgttgct gccctgcgga attgacaagt tccgaggggt agagtttgtg
2281 tgttgcccac tggctgaaga aagtgacaat gtggattctg ctgatgcgga ggaggatgac
2341 tcggatgtct ggtggggcgg agcagacaca gactatgcag atgggagtga agacaaagta
2401 gtagaagtag cagaggagga agaagtggct gaggtggaag aagaagaagc cgatgatgac
2461 gaggacgatg aggatggtga tgaggtagag gaagaggctg aggaacccta cgaagaagcc
2521 acagagagaa ccaccagcat tgccaccacc accaccacca ccacagagtc tgtggaagag
2581 gtggttcgag ttcctacaac agcagccagt acccctgatg ccgttgacaa gtatctcgag
2641 acacctgggg atgagaatga acatgcccat ttccagaaag ccaaagagag gcttgaggcc
2701 aagcaccgag agagaatgtc ccaggtcatg agagaatggg aagaggcaga acgtcaagca
2761 aagaacttgc ctaaagctga taagaaggca gttatccagc atttccagga gaaagtggaa
2821 tctttggaac aggaagcagc caacgagaga cagcagctgg tggagacaca catggccaga
2881 gtggaagcca tgctcaatga ccgccgccgc ctggccctgg agaactacat caccgctctg
2941 caggctgttc ctcctcggcc tcgtcacgtg ttcaatatgc taaagaagta tgtccgcgca
3001 gaacagaagg acagacagca caccctaaag catttcgagc atgtgcgcat ggtggatccc
3061 aagaaagccg ctcagatccg gtcccaggtt atgacacacc tccgtgtgat ttatgagcgc
3121 atgaatcagt ctctctccct gctctacaac gtgcctgcag tggccgagga gattcaggat
3181 gaagttgatg agctgcttca gaaagagcaa aactattcag atgacgtctt ggccaacatg
3241 attagtgaac caaggatcag ttacggaaac gatgctctca tgccatcttt gaccgaaacg
3301 aaaaccaccg tggagctcct tcccgtgaat ggagagttca gcctggacga tctccagccg
3361 tggcattctt ttggggctga ctctgtgcca gccaacacag aaaacgaagt tgagcctgtt
3421 gatgcccgcc ctgctgccga ccgaggactg accactcgac caggttctgg gttgacaaat
3481 atcaagacgg aggagatctc tgaagtgaat ctggatgcag aattccgaca tgactcagga
3541 tatgaagttc atcatcaaaa attggtgttc tttgcagaag atgtgggttc aaacaaaggt
3601 gcaatcattg gactcatggt gggcggtgtt gtcatagcga cagtgatctt catcaccttg
3661 gtgatgctga agaagaaaca gtacacatcc attcatcatg gtgtggtgga ggttgacgcc
3721 gctgtcaccc cagaggagcg ccacctgtcc aagatgcagc agaacggcta cgaaaatcca
3781 acctacaagt tctttgagca gatgcagaac taggatatcg cggccgcctc gagagatctt
3841 tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc
3901 taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc
3961 ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt
4021 ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat
4081 cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga
4141 ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt
4201 tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct
4261 gtccctcttc tcttatggag atccctcgac ctgcagccca agcttggcgt aatcatggtc
4321 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
4381 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
4441 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat
4501 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
4561 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
4621 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
4681 tgcaaaaagc taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
4741 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
4801 tcaatgtatc ttatcatgtc tggatccgct gcattaatga atcggccaac gcgcggggag
4861 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
4921 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
4981 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
5041 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
5101 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
5161 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
5221 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
5281 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
5341 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
5401 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
5461 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
5521 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
5581 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
5641 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
5701 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
5761 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
5821 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
5881 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
5941 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
6001 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
6061 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
6121 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
6181 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
6241 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
6301 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
6361 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
6421 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
6481 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
6541 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
6601 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
6661 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
6721 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
6781 ggttccgcgc acatttcccc gaaaagtgcc acctgg
//
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
- 上一篇：pRK5-EGFP-Tau
- 下一篇：pEF-hSEMA4C-Fc
